或搭建多片段脱氧核糖核酸组件 NEB ^®有解决之道高质量试剂可供所有工作流使用,包括常用脱氧核糖核酸汇编法等 NEBULEDER系统^®HiFi脱氧核糖核酸汇编并 NEBridge^®金门大会.并提供自动化、网站引导变异学解决方案以及你最喜爱的限制酶、ligase或合格细胞产品满怀信心寻找克隆时,想一想NEB

脱氧核糖核酸组装和克隆

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首页 bob游戏手机版用法指南回归基础知识:重要事物在净化粒子和脱氧核糖核酸碎片时记住

回归基础知识:重要事物在净化粒子和脱氧核糖核酸碎片时记住

脱氧核糖核酸绑定高盐条件西里卡并释放低盐条件西里卡
正因如此绑定缓冲区使用盐类和脱氧核糖核酸缓冲不含盐
大片比较难理解,因为它们与列矩阵绑定得更紧
因此,从列中发布它们更具挑战性热明市elude缓冲区并允许排解缓冲置列可提高排解效率
执行时模模小型预科准备更多细胞不一定等同多图解DNA恢复
使用过多细胞文化可阻塞列并降低绑定效率,实际上降低恢复效率通常有理想文化OD范围,最优选择给定列看我们的d's和n'tminipreps和我们净化低拷贝Plamids指南获取更多实用技巧
低拷贝粒子工作时,通常会看到粒子预览中的残留gDNA
Exonclease VgDNA可选择消化线性脱氧核糖核酸并保持圆形脱氧核酸完整查查这个应用注解获取更多信息
残留乙醇流出法中,使用含有保留环的列时常见化(塑料环置住膜)。
bob软件苹果版溶液中的乙醇会干扰下游应用,并还可能导致样本加载凝胶道时浮出水井monarch脱氧核糖核酸清理列并miniprep列设计时不保留环,消除移位污染风险
常见原因人执行时低产量gel提取显示它们不正确释放Agarose凝胶上所有DNA
使用推荐量很重要消散缓冲区并保证agarose完全熔化前继续更多Gel提取技巧见六大提示完美Gel采掘.

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